Effects of Pluronic F68 on Manganese peroxidase production by pelletized Phanerochaete chrysosporium.
Identifieur interne : 000511 ( Main/Exploration ); précédent : 000510; suivant : 000512Effects of Pluronic F68 on Manganese peroxidase production by pelletized Phanerochaete chrysosporium.
Auteurs : Zhi-Min Li [République populaire de Chine] ; Yan Liu ; Zhan-You Chi ; Shu-Lin ChenSource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2011.
Descripteurs français
- KwdFr :
- Bioréacteurs (microbiologie), Concentration en ions d'hydrogène (MeSH), Facteurs temps (MeSH), Fermentation (MeSH), Peroxidases (biosynthèse), Phanerochaete (enzymologie), Phanerochaete (métabolisme), Poloxamère (composition chimique), Polysorbates (composition chimique), Polyéthylène glycols (composition chimique).
- MESH :
- biosynthèse : Peroxidases.
- composition chimique : Poloxamère, Polysorbates, Polyéthylène glycols.
- enzymologie : Phanerochaete.
- microbiologie : Bioréacteurs.
- métabolisme : Phanerochaete.
- Concentration en ions d'hydrogène, Facteurs temps, Fermentation.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Peroxidases.
- chemical , chemistry : Poloxamer, Polyethylene Glycols, Polysorbates.
- enzymology : Phanerochaete.
- metabolism : Phanerochaete.
- microbiology : Bioreactors.
- Fermentation, Hydrogen-Ion Concentration, Time Factors.
Abstract
In this study, a new process was developed for manganese peroxidase (MnP) production by Phanerochaete chrysosporium under an agitated and aerated cultivation condition. It was found that change of the inoculum from spore suspension to pellets resulted in enhanced MnP production of 200 U/L in rotated shake flasks. Several additives, including Pluronic F68, Tween 80, and PEG8000, significantly increased the enzyme production. With an optimal concentration in 125 mL flasks, Pluronic F68 increased MnP productivity by 180%. Moreover, successful enzyme production was achieved in a 5-L fermentor at an agitation speed of 300 rpm with the addition of 0.1% Pluronic F68.
DOI: 10.1007/s12010-010-9150-6
PubMed: 21234704
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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sortKey="Chen, Shu Lin" sort="Chen, Shu Lin" uniqKey="Chen S" first="Shu-Lin" last="Chen">Shu-Lin Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21234704</idno><idno type="pmid">21234704</idno><idno type="doi">10.1007/s12010-010-9150-6</idno><idno type="wicri:Area/Main/Corpus">000515</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000515</idno><idno type="wicri:Area/Main/Curation">000515</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000515</idno><idno type="wicri:Area/Main/Exploration">000515</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of Pluronic F68 on Manganese peroxidase production by pelletized Phanerochaete chrysosporium.</title><author><name sortKey="Li, Zhi Min" sort="Li, Zhi Min" uniqKey="Li Z" first="Zhi-Min" last="Li">Zhi-Min Li</name><affiliation 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qualifier="microbiology" xml:lang="en"><term>Bioreactors</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Fermentation</term><term>Hydrogen-Ion Concentration</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term><term>Facteurs temps</term><term>Fermentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, a new process was developed for manganese peroxidase (MnP) production by Phanerochaete chrysosporium under an agitated and aerated cultivation condition. It was found that change of the inoculum from spore suspension to pellets resulted in enhanced MnP production of 200 U/L in rotated shake flasks. Several additives, including Pluronic F68, Tween 80, and PEG8000, significantly increased the enzyme production. With an optimal concentration in 125 mL flasks, Pluronic F68 increased MnP productivity by 180%. Moreover, successful enzyme production was achieved in a 5-L fermentor at an agitation speed of 300 rpm with the addition of 0.1% Pluronic F68.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21234704</PMID><DateCompleted><Year>2011</Year><Month>08</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>164</Volume><Issue>4</Issue><PubDate><Year>2011</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Effects of Pluronic F68 on Manganese peroxidase production by pelletized Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>487-96</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-010-9150-6</ELocationID><Abstract><AbstractText>In this study, a new process was developed for manganese peroxidase (MnP) production by Phanerochaete chrysosporium under an agitated and aerated cultivation condition. It was found that change of the inoculum from spore suspension to pellets resulted in enhanced MnP production of 200 U/L in rotated shake flasks. Several additives, including Pluronic F68, Tween 80, and PEG8000, significantly increased the enzyme production. With an optimal concentration in 125 mL flasks, Pluronic F68 increased MnP productivity by 180%. 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